Human immunodeficiency virus type 1 capsid protein is a substrate of the retroviral proteinase while integrase is resistant toward proteolysis.
Virology
; 310(1): 16-23, 2003 May 25.
Article
en En
| MEDLINE
| ID: mdl-12788626
The capsid protein of human immunodeficiency virus type 1 was observed to undergo proteolytic cleavage in vitro when viral lysate was incubated in the presence of dithiothreitol at acidic pH. Purified HIV-1 capsid protein was also found to be a substrate of the viral proteinase in a pH-dependent manner; acidic pH (<7) was necessary for cleavage, and decreasing the pH toward 4 increased the degree of processing. Based on N-terminal sequencing of the cleavage products, the capsid protein was found to be cleaved at two sites, between residues 77 and 78 as well as between residues 189 and 190. Oligopeptides representing these cleavage sites were also cleaved at the expected peptide bonds. The presence of cyclophilin A decreased the degree of capsid protein processing. Unlike the capsid protein, integrase was found to be resistant toward proteolysis in good agreement with its presence in the preintegration complex.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteasa del VIH
/
VIH-1
/
Integrasa de VIH
/
Proteínas de la Cápside
Idioma:
En
Revista:
Virology
Año:
2003
Tipo del documento:
Article
País de afiliación:
Hungria
Pais de publicación:
Estados Unidos