Cloning of canine hematopoietic cells in vitro. / Klonirovanie krovetvornykh kletok sobak in vitro
Biull Eksp Biol Med
; 81(4): 472-5, 1976.
Article
en Ru
| MEDLINE
| ID: mdl-1276454
A modified cloning method of the agar culture of canine bone marrow cells was described. A high efficiency of colony formation was seen only after addition to the agar medium of the colony-stimulating activity (CSA) from different sources. Dog serum in a 20% concentration was used in this case. With the optimal CSA concentration there was seen a linear relationship between the number of explanted cells and the number of produced colonies. This method is suitable for determination of committed granulocyte precursor cells, as well as for the study of potential humoral regulators of granulocytopoiesis in dogs.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Médula Ósea
/
Células de la Médula Ósea
/
División Celular
/
Células Clonales
Límite:
Animals
Idioma:
Ru
Revista:
Biull Eksp Biol Med
Año:
1976
Tipo del documento:
Article
Pais de publicación:
Rusia