Purification and characterization of an endochitinase produced by Colletotrichum gloeosporioides.

Souza, R F; Gomes, R C; Coelho, R R R; Alviano, C S; Soares, R M A

Souza, R F; Gomes, R C; Coelho, R R R; Alviano, C S; Soares, R M A.

Afiliación

Souza RF; Departamento de Microbiologia Geral, Instituto de Microbiologia Prof Paulo de Góes (IMPPG), Centro de Ciências da Saúde, Universidade do Brasil, Ilha do Fundão, CEP 21941-590, Rio de Janeiro, RJ, Brazil.

FEMS Microbiol Lett ; 222(1): 45-50, 2003 May 16.

Article en En | MEDLINE | ID: mdl-12757945

RESUMEN

The phytopathogenic fungus Colletotrichum gloeosporioides was analyzed for chitinase activity, the best production occurring at the fourth day. A 43 kDa endochitinase with specific activity of 413 U microg(-1) protein was purified corresponding to a 75% yield. The optima of temperature and pH for the enzyme were 50 degrees C and pH 7.0, respectively. The enzyme showed a high stability at 50 degrees C and pH 7.0. Values of pH from 5.0 up to 7.0 gave, at least, 50% of maximum activity, suggesting a biotechnological application. Further studies are in progress to determine the possible use of this endochitinase in biological control.

Asunto(s)

Quitina/metabolismo; Quitinasas/aislamiento & purificación; Quitinasas/metabolismo; Colletotrichum/enzimología; Carbono/metabolismo; Quitinasas/análisis; Coloides; Medios de Cultivo; Electroforesis en Gel de Poliacrilamida; Concentración de Iones de Hidrógeno; Cinética

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Quitina / Quitinasas / Colletotrichum Idioma: En Revista: FEMS Microbiol Lett Año: 2003 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido

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