New gastric epithelial cell lines from mice transgenic for temperature-sensitive simian virus 40 large T antigen show distinct types of cell differentiation.
Digestion
; 67(1-2): 71-81, 2003.
Article
en En
| MEDLINE
| ID: mdl-12743444
AIM: To develop conditionally immortalized gastric mucosal cell lines that show distinct types of cell differentiation from transgenic mice harboring temperature-sensitive simian virus 40 (tsSV40) large T antigen. METHODS: Gastric mucosal cells from the transgenic mice were cultured at a permissive temperature (33 degrees C), and proliferative cells were then cloned by colony formation. RESULTS: Eight gastric cell lines showed epithelial-like morphology and grew at 33 degrees C. Three different types of the cell lines have been established: (1) MGE12-1, MGE3-2, and MGE509 cells expressing mRNAs for pit cell markers (gastric mucin and cathepsin E); (2) MGE02, MGE503, and MGE511 cells expressing mRNAs for pit and zymogenic (pepsinogen F) cell markers, and (3) MGE507 and MGE727 cells expressing mRNAs for pit, zymogenic, and parietal (H,K-ATPase alpha-subunit) cell markers. Moreover, the TaqMan assay showed that mRNA levels of mucin, H,K-ATPase alpha-subunit, and pepsinogen F were influenced by nonpermissive temperature (39 degrees C) in MGE503 and MGE727 cells. CONCLUSION: These gastric epithelial cell lines seem to reflect different stages of development of gastric mucosal cells.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Diferenciación Celular
/
Antígenos Transformadores de Poliomavirus
/
Células Epiteliales
/
Mucosa Gástrica
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Revista:
Digestion
Año:
2003
Tipo del documento:
Article
País de afiliación:
Japón
Pais de publicación:
Suiza