Freeze-fracture electron microscopy of lipid membranes on colloidal polyelectrolyte multilayer coated supports.
Biomacromolecules
; 4(3): 808-14, 2003.
Article
en En
| MEDLINE
| ID: mdl-12741802
Lipid membranes were assembled on polyelectrolyte (PE)-coated colloidal particles. The assembly was studied by means of confocal microscopy, flow cytometry, scanning force microscopy, and freeze-fracture electron microscopy. A homogeneous lipid coverage was established within the limits of optical resolution. Flow cytometry showed that the lipid coverage was uniform. Freeze-fracture electron microscopy revealed that the lipid was adsorbed as a bilayer, which closely followed the surface profile of the polyelectrolyte support. Additional adsorption of polyelectrolyte layers on top of the lipid bilayer introduced inhomogeneities as evident from jumps in the fracture plane. Characteristic lipid multilayers have not been seen with freeze-fracture electron microscopy.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Polímeros
/
Microscopía Electrónica
/
Técnica de Fractura por Congelación
/
Lípidos
/
Membranas Artificiales
Idioma:
En
Revista:
Biomacromolecules
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2003
Tipo del documento:
Article
País de afiliación:
Alemania
Pais de publicación:
Estados Unidos