Leptin induces mitogenic effect on human choriocarcinoma cell line (JAr) via MAP kinase activation in a glucose-dependent fashion.
Placenta
; 24(4): 385-91, 2003 Apr.
Article
en En
| MEDLINE
| ID: mdl-12657513
Leptin and glucose effect on cell growth has been investigated in the JAr human choriocarcinoma cells. When JAr cells were cultured in the presence of 6m M glucose (LG), proliferation and thymidine incorporation were induced by serum but not by leptin. At variance, at 25m M glucose (HG), proliferation and thymidine incorporation were stimulated by leptin and serum to a comparable extent. HG culturing also enhanced leptin-stimulated insulin receptor substrate 1 (IRS1) and MAPK phosphorylation. Blockage of MAPK activity with PD98059 caused an inhibition of glucose- and leptin-dependent thymidine incorporation. At variance with HG conditions no effects were observed in cells cultured in 6m M glucose upon treatment with PD98059. Neither glucose nor leptin determined a modification in leptin receptors total content. In this study, we provide evidence that in placental cells, leptin, similarly to that observed with insulin, stimulates cell proliferation by inducing the IRS1/MAPK pathway in a glucose-dependent fashion.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Trofoblastos
/
Sustancias de Crecimiento
/
Leptina
/
Proteínas Quinasas Activadas por Mitógenos
/
Glucosa
Límite:
Female
/
Humans
Idioma:
En
Revista:
Placenta
Año:
2003
Tipo del documento:
Article
País de afiliación:
Italia
Pais de publicación:
Países Bajos