Transferrin-dependent expression of TbpA by Histophilus ovis involves a poly G tract within tbpA.
FEMS Microbiol Lett
; 220(1): 95-8, 2003 Mar 14.
Article
en En
| MEDLINE
| ID: mdl-12644233
A poly G tract in tbpA of Histophilus ovis strain 3384Y was suspected of being responsible for the transferrin (Tf)-dependent expression of TbpA. The region encompassing the poly G tract was amplified using DNA from H. ovis strains 9L and 3384Y grown under iron-replete conditions and under iron-restricted conditions in the presence of bovine Tf. Sequence analysis of the amplification products revealed that regardless of the growth conditions, the poly G tract in strain 9L contained eight Gs, a situation that maintains the correct reading frame of the gene. Similarly, the poly G tract in strain 3384Y contained eight Gs when the organisms were grown under iron-restricted conditions in the presence of bovine Tf but when grown under iron-replete conditions, the poly G tract contained nine Gs resulting in a frame shift and the introduction of a premature stop codon. It is concluded that the Tf-dependent expression of TbpA in H. ovis strain 3384Y is due to a form of phase variation.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Poli G
/
ADN Bacteriano
/
Transferrina
/
Regulación Bacteriana de la Expresión Génica
/
Pasteurellaceae
/
Proteína A de Unión a Transferrina
Límite:
Animals
Idioma:
En
Revista:
FEMS Microbiol Lett
Año:
2003
Tipo del documento:
Article
Pais de publicación:
Reino Unido