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Requirement of different signaling pathways mediated by insulin-like growth factor-I receptor for proliferation, invasion, and VPF/VEGF expression in a pancreatic carcinoma cell line.
Zeng, Huiyan; Datta, Kaustubh; Neid, Matthias; Li, Jinping; Parangi, Sareh; Mukhopadhyay, Debabrata.
Afiliación
  • Zeng H; Department of Pathology, RN270H, Beth Israel Deaconess Medical Center and Harvard Medical School, 99 Brookline Avenue, Boston, MA 02215, USA.
Biochem Biophys Res Commun ; 302(1): 46-55, 2003 Feb 28.
Article en En | MEDLINE | ID: mdl-12593846
Several oncogenes and growth factors are found to be mutated and overexpressed in adenocarcinoma of the pancreas, and may correlate with its highly aggressive nature. Insulin-like growth factor (IGF-I) and its receptor (IGF-IR) are highly expressed in this tumor type. We examined the IGF-IR-mediated signaling pathways in relation to cell proliferation, invasiveness, and expression pattern of vascular permeability factor/vascular endothelial growth factor (VPF/VEGF) in the pancreatic cancer line ASPC-1. Our findings show that IGF-IR is an important growth factor receptor for cell proliferation and invasion, and VPF/VEGF expression in ASPC-1. Further experiments indicate that IGF-IR mediates different signaling pathways to execute its functions. Activation of Ras by IGF-IR was found to be required for the cell invasion. On the other hand Src activation through IGF-IR is required for the cell proliferation, invasion, and also VPF/VEGF expression. Taken together, our data indicate the importance of IGF-IR in growth and invasiveness of the pancreatic cancer cell lines and also point out the multiple signaling pathways channeled through this receptor.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Pancreáticas / Transducción de Señal / División Celular / Factores de Crecimiento Endotelial / Linfocinas / Receptor IGF Tipo 1 / Péptidos y Proteínas de Señalización Intercelular / Invasividad Neoplásica Límite: Humans Idioma: En Revista: Biochem Biophys Res Commun Año: 2003 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Pancreáticas / Transducción de Señal / División Celular / Factores de Crecimiento Endotelial / Linfocinas / Receptor IGF Tipo 1 / Péptidos y Proteínas de Señalización Intercelular / Invasividad Neoplásica Límite: Humans Idioma: En Revista: Biochem Biophys Res Commun Año: 2003 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos