RNase L mediates transient control of the interferon response through modulation of the double-stranded RNA-dependent protein kinase PKR.
J Biol Chem
; 278(22): 20124-32, 2003 May 30.
Article
en En
| MEDLINE
| ID: mdl-12582177
The transient control of diverse biological responses that occurs in response to varied forms of stress is often a highly regulated process. During the interferon (IFN) response, translational repression due to phosphorylation of eukaryotic initiation factor 2alpha, eIF2alpha, by the double-stranded RNA-dependent protein kinase, PKR, constitutes a means of inhibiting viral replication. Here we show that the transient nature of the IFN response against acute viral infections is regulated, at least in part, by RNase L. During the IFN antiviral response in RNase L-null cells, PKR mRNA stability was enhanced, PKR induction was increased, and the phosphorylated form of eIF2alpha appeared with extended kinetics compared with similarly treated wild type cells. An enhanced IFN response in RNase L-null cells was also demonstrated by monitoring inhibition of viral protein synthesis. Furthermore, ectopic expression of RNase L from a plasmid vector prevented the IFN induction of PKR. These results suggest a role for RNase L in the transient control of the IFN response and possibly of other cytokine and stress responses.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
ARN Bicatenario
/
Interferones
/
EIF-2 Quinasa
/
Endorribonucleasas
Límite:
Animals
Idioma:
En
Revista:
J Biol Chem
Año:
2003
Tipo del documento:
Article
País de afiliación:
Arabia Saudita
Pais de publicación:
Estados Unidos