[Mutation analysis of the tumor suppressor gene PTEN/MMAC1/TEP1 in human hepatocellular carcinoma].
Shi Yan Sheng Wu Xue Bao
; 33(3): 223-7, 2000 Sep.
Article
en Zh
| MEDLINE
| ID: mdl-12549040
PTEN/MMAC1/TEP1 is a tumor suppressor gene. Its mutation has been found in several different types of human cancers. 34 primary human hepatocellular carcinomas have been examined for mutations in exon 5 and exon 8 of the PTEN gene. Exon 5 and exon 8 were amplified by polymerase chain reaction (PCR) with intronic primers and subjected to single strand conformation polymorphism (SSCP) analysis. SSCPs were found in 4 of the 34 hepatocellular carcinomas analyzed. Direct sequencing of the PCR products identified single base-pair substitutions in the four tumor DNA samples, two in intron 4 and two in exon 8. One of the base-pair substitution in exon 8 is a missense mutation, which changed codon 304 of PTEN protein from Cys to Gly. These data suggest that PTEN may be involved in the carcinogenesis and development of hepatocellular carcinoma.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Genes Supresores de Tumor
/
Carcinoma Hepatocelular
/
Neoplasias Hepáticas
/
Mutación
Idioma:
Zh
Revista:
Shi Yan Sheng Wu Xue Bao
Año:
2000
Tipo del documento:
Article
Pais de publicación:
China