Carbohydrate ingestion influences skeletal muscle cytokine mRNA and plasma cytokine levels after a 3-h run.

Nieman, D C; Davis, J M; Henson, D A; Walberg-Rankin, J; Shute, M; Dumke, C L; Utter, A C; Vinci, D M; Carson, J A; Brown, A; Lee, W J; McAnulty, S R; McAnulty, L S

Nieman, D C; Davis, J M; Henson, D A; Walberg-Rankin, J; Shute, M; Dumke, C L; Utter, A C; Vinci, D M; Carson, J A; Brown, A; Lee, W J; McAnulty, S R; McAnulty, L S.

Afiliación

Nieman DC; Department of Health, Fischer Hamilton/Nycom Biochemistry Laboratory, Appalachian State University, Boone, North Carolina 28608, USA. niemandc@appstate.edu

J Appl Physiol (1985) ; 94(5): 1917-25, 2003 May.

Article en En | MEDLINE | ID: mdl-12533503

RESUMEN

Sixteen experienced marathoners ran on treadmills for 3 h at approximately 70% maximal oxygen consumption (Vo(2 max)) on two occasions while receiving 1 l/h carbohydrate (CHO) or placebo (Pla) beverages. Blood and vastus lateralis muscle biopsy samples were collected before and after exercise. Plasma was analyzed for IL-6, IL-10, IL-1 receptor agonist (IL-1ra), IL-8, cortisol, glucose, and insulin. Muscle was analyzed for glycogen content and relative gene expression of 13 cytokines by using real-time quantitative RT-PCR. Plasma glucose and insulin were higher, and cortisol, IL-6, IL-10, and IL-1ra, but not IL-8, were significantly lower postexercise in CHO vs. Pla. Change in muscle glycogen content did not differ between CHO and Pla (P = 0.246). Muscle cytokine mRNA content was detected preexercise for seven cytokines in this order (highest to lowest): IL-15, TNF-alpha, IL-8, IL-1beta, IL-12p35, IL-6, and IFN-gamma. After subjects ran for 3 h, gene expression above prerun levels was measured for five of these cytokines: IL-1beta, IL-6, and IL-8 (large increases), and IL-10 and TNF-alpha (small increases). The increase in mRNA (fold difference from preexercise) was attenuated in CHO (15.9-fold) compared with Pla (35.2-fold) for IL-6 (P = 0.071) and IL-8 (CHO, 7.8-fold; Pla, 23.3-fold; P = 0.063). CHO compared with Pla beverage ingestion attenuates the increase in plasma IL-6, IL-10, and IL-1ra and gene expression for IL-6 and IL-8 in athletes running 3 h at 70% Vo(2 max) despite no differences in muscle glycogen content.

Asunto(s)

Citocinas/biosíntesis; Citocinas/sangre; Carbohidratos de la Dieta/farmacología; Músculo Esquelético/metabolismo; ARN Mensajero/biosíntesis; Carrera/fisiología; Adulto; Recuento de Células Sanguíneas; Glucemia/metabolismo; Citocinas/genética; ADN Complementario/biosíntesis; ADN Complementario/genética; Femenino; Expresión Génica; Glucógeno/metabolismo; Hormonas/sangre; Humanos; Hidrocortisona/sangre; Inmunoglobulina A/análisis; Inmunoglobulina A/metabolismo; Recuento de Leucocitos; Masculino; Persona de Mediana Edad; Músculo Esquelético/efectos de los fármacos; Consumo de Oxígeno/efectos de los fármacos; Consumo de Oxígeno/fisiología; ARN Mensajero/genética; Saliva/química; Saliva/inmunología

Buscar en Google

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Carrera / ARN Mensajero / Carbohidratos de la Dieta / Citocinas / Músculo Esquelético Tipo de estudio: Clinical_trials Límite: Adult / Female / Humans / Male / Middle aged Idioma: En Revista: J Appl Physiol (1985) Asunto de la revista: FISIOLOGIA Año: 2003 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos

Buscar en Google

Añadir a Mi BVS

Imprimir

XML

PubMed Links