Purification and properties of a transglutaminase produced by a Bacillus circulans strain isolated from the Amazon environment.
Biotechnol Appl Biochem
; 37(Pt 3): 295-9, 2003 Jun.
Article
en En
| MEDLINE
| ID: mdl-12529180
A new microbial transglutaminase (EC 2.3.2.13) from a Bacillus circulans strain isolated from the aquatic Amazonian environment was purified and characterized. Enzyme purification started with (NH(4))(2)SO(4) 'salting out' and proceeded with liquid chromatography on Q-Sepharose FF and octyl-Sepharose 4 FF. The purification factor was approx. 150-fold with a yield of 32%. The enzyme's molecular mass was estimated as 45000 Da on SDS/PAGE. The purified transglutaminase had an optimum temperature of 47 degrees C, the optimum pH of the reaction was 7 and it presented no calcium-dependent activity.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Bacillus
/
Transglutaminasas
/
Cromatografía
/
Reactores Biológicos
País/Región como asunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Biotechnol Appl Biochem
Asunto de la revista:
BIOQUIMICA
/
BIOTECNOLOGIA
Año:
2003
Tipo del documento:
Article
País de afiliación:
Brasil
Pais de publicación:
Estados Unidos