An improved method for recovering rabies virus from cloned cDNA.
J Virol Methods
; 107(2): 229-36, 2003 Feb.
Article
en En
| MEDLINE
| ID: mdl-12505638
A new system for recovery of rabies virus from cDNA plasmid, the transcription of which was driven by cellular RNA polymerase II, was developed. The plasmid contains full-length viral cDNA flanked by hammerhead ribozyme and hepatitis delta ribozyme sequences, arranged downstream of the cytomegalovirus (CMV) promotor. Transfection with the full-length cDNA plasmid together with helper plasmids encoding viral N, P, and L proteins without supply of T7 RNA polymerase produced a recombinant rabies virus in several cell lines. The efficiency of recovery between the conventional T7 promotor system and the new CMV promotor system was compared using these plasmid constructs. The newly established system is applicable to various cell lines and allows rapid and efficient generation of recombinant rabies virus.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Virus de la Rabia
/
Clonación Molecular
/
ADN Complementario
Tipo de estudio:
Evaluation_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
J Virol Methods
Año:
2003
Tipo del documento:
Article
País de afiliación:
Japón
Pais de publicación:
Países Bajos