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Erythrocyte CR1 expression level does not correlate with a HindIII restriction fragment length polymorphism in Africans; implications for studies on malaria susceptibility.
Rowe, J A; Raza, A; Diallo, D A; Baby, M; Poudiougo, B; Coulibaly, D; Cockburn, I A; Middleton, J; Lyke, K E; Plowe, C V; Doumbo, O K; Moulds, J M.
Afiliación
  • Rowe JA; Institute of Cell, Animal and Population Biology, University of Edinburgh, Edinburgh, UK. Alex.Rowe@ed.ac.uk
Genes Immun ; 3(8): 497-500, 2002 Dec.
Article en En | MEDLINE | ID: mdl-12486610
Complement receptor 1 (CR1) expression level on erythrocytes is genetically determined, and in Caucasian populations is linked to high (H) and low (L) expression alleles identified by a HindIII restriction fragment length polymorphism (RFLP). Erythrocyte CR1 may be an important factor in determining malaria susceptibility, as low expression of CR1 reduces the rosetting of uninfected erythrocytes with Plasmodium falciparum-infected cells, a process that contributes to malaria pathogenesis. Prior to studying CR1 expression and malaria susceptibility, we have investigated whether the quantity of erythrocyte CR1 correlates with the H and L alleles in an African population. Mean erythrocyte CR1 in 149 Malian adults was 415 molecules per cell, which is comparable to Caucasian populations; however, there was no relationship between erythrocyte CR1 level and genotype for the HindIII RFLP (mean CR1 per erythrocyte HH = 414, HL = 419 and LL = 403, P > 0.1, Student's t-test). The conclusions of a previous study of erythrocyte CR1 expression level and malaria susceptibility in West Africa that was based on HindIII RFLP genotyping may therefore need to be re-evaluated.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plasmodium falciparum / Polimorfismo de Longitud del Fragmento de Restricción / Metiltransferasa de ADN de Sitio Específico (Adenina Especifica) / Malaria Falciparum / Receptores de Complemento 3b / Predisposición Genética a la Enfermedad / Eritrocitos Tipo de estudio: Prognostic_studies Límite: Animals / Humans País/Región como asunto: Africa Idioma: En Revista: Genes Immun Asunto de la revista: ALERGIA E IMUNOLOGIA / BIOLOGIA MOLECULAR Año: 2002 Tipo del documento: Article Pais de publicación: Reino Unido
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plasmodium falciparum / Polimorfismo de Longitud del Fragmento de Restricción / Metiltransferasa de ADN de Sitio Específico (Adenina Especifica) / Malaria Falciparum / Receptores de Complemento 3b / Predisposición Genética a la Enfermedad / Eritrocitos Tipo de estudio: Prognostic_studies Límite: Animals / Humans País/Región como asunto: Africa Idioma: En Revista: Genes Immun Asunto de la revista: ALERGIA E IMUNOLOGIA / BIOLOGIA MOLECULAR Año: 2002 Tipo del documento: Article Pais de publicación: Reino Unido