Expression and activity of a multixenobiotic resistance system in the Pacific oyster Crassostrea gigas.
Mar Environ Res
; 54(3-5): 455-9, 2002.
Article
en En
| MEDLINE
| ID: mdl-12408601
The multidrug resistance (MDR) mechanism corresponds to a defence system relying on the expression of high molecular membrane proteins that can actively lower the intracellular concentration of a wide variety of toxins, thus maintaining them below their toxic level. Using RT-PCR, expression levels of a gene belonging to the class I of mammalian mdr genes, has been assessed in different developmental stages of the oyster Crassostrea gigas. While no expression was found in the oocyte or the trocophore stage, a rise of mRNA content was observed from the veliger stage to the juvenile stage, thus indicating the induction of the system as the animal is developing in the environment. The incubation of gill fragments in the dye rhodamine B and subsequent measurements of intracellular fluorescence using a microplate reader indicates that the system can effectively decrease the accumulation of the test compound in a competitive manner with known inhibitors or environmental contaminants as observed in vertebrate cells. The oyster MXR system is thus becoming active in adult oyster and could be of importance in environmentally contaminated areas.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ostreidae
/
Xenobióticos
/
Regulación de la Expresión Génica
/
Resistencia a Múltiples Medicamentos
/
Genes MDR
Límite:
Animals
Idioma:
En
Revista:
Mar Environ Res
Asunto de la revista:
BIOLOGIA
/
SAUDE AMBIENTAL
/
TOXICOLOGIA
Año:
2002
Tipo del documento:
Article
País de afiliación:
Francia
Pais de publicación:
Reino Unido