Chondrocyte recovery in cryopreserved porcine articular cartilage after bone carrier alteration.
Cryo Letters
; 23(4): 263-8, 2002.
Article
en En
| MEDLINE
| ID: mdl-12391487
In order to investigate the consequences on the distribution of cell recovery through a cross-section of articular cartilage, the pathway for ice nucleation and diffusion of water and solutes in porcine osteochondral tissue was altered by drilling a 2mm diameter hole through the subchondral bone to the base of the cartilage. Samples equilibrated with 1M dimethyl sulfoxide were cooled at 1 C/min to -30 degrees C then stored in liquid nitrogen. A significant increase in chondrocyte recovery was documented when compared to samples cryopreserved without holes (48.3 percent vs 28.6 percent, P=0.003). The most significant change due to bone base modification was an increase in recovery in the middle section of the cartilage. These results provide insight into mechanisms of cryoinjury in tissue systems.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Cartílago Articular
/
Criopreservación
/
Condrocitos
Límite:
Animals
Idioma:
En
Revista:
Cryo Letters
Asunto de la revista:
BIOLOGIA
/
QUIMICA
Año:
2002
Tipo del documento:
Article
Pais de publicación:
Reino Unido