Direct visualization by confocal fluorescent microscopy of the permeation of myristoylated peptides through the cell membrane.
IUBMB Life
; 54(1): 33-6, 2002 Jul.
Article
en En
| MEDLINE
| ID: mdl-12387573
To study the permeability through the cellular membrane of synthetic peptides containing an hydrophobic moiety, we used a 13-mer myristoylated peptide labeled with a N-terminal fluorescent probe. After 2 h of incubation, the subcellular distribution was analyzed in intact chromaffin cells by confocal fluorescent microscopy. Our results demonstrate that myristoylated peptides diffuse into intact cells, showing an heterogeneous distribution, but they do not reach the cellular nucleous, at least during the time range used.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Péptidos
/
Permeabilidad de la Membrana Celular
/
Microscopía Confocal
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Células Cromafines
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Microscopía Fluorescente
Límite:
Animals
Idioma:
En
Revista:
IUBMB Life
Asunto de la revista:
BIOLOGIA MOLECULAR
/
BIOQUIMICA
Año:
2002
Tipo del documento:
Article
País de afiliación:
España
Pais de publicación:
Reino Unido