HPV DNA detection by in situ hybridization with catalyzed signal amplification on thin-layer cervical smears.

Samama, Brigitte; Plas-Roser, Salomé; Schaeffer, Christiane; Chateau, Danielle; Fabre, Michel; Boehm, Nelly

Samama, Brigitte; Plas-Roser, Salomé; Schaeffer, Christiane; Chateau, Danielle; Fabre, Michel; Boehm, Nelly.

Afiliación

Samama B; Institut d'Histologie, Faculté de Médecine, Strasbourg, France. Brigitte.Samama@medecine.u-strasbg.fr

J Histochem Cytochem ; 50(10): 1417-20, 2002 Oct.

Article en En | MEDLINE | ID: mdl-12364574

RESUMEN

Thin layer-based technology in cervical cancer screening now allows both Papanicolaou staining and HPV testing on the same sample. Here, we show that in situ hybridization with catalyzed reporter deposition is a powerful HPV detection method when applied on thin-layer cervical smears, allowing distinction between two staining patterns suggestive of two different physical states of HPV DNA, where diffuse signals are suggestive of episomes and punctate signals are suggestive of viral DNA integration.

Asunto(s)

ADN Viral/análisis; Papillomaviridae/genética; Neoplasias del Cuello Uterino/diagnóstico; Femenino; Humanos; Hibridación in Situ/métodos; Prueba de Papanicolaou; Neoplasias del Cuello Uterino/patología; Neoplasias del Cuello Uterino/virología; Frotis Vaginal

Buscar en Google

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Papillomaviridae / ADN Viral / Neoplasias del Cuello Uterino Tipo de estudio: Diagnostic_studies Límite: Female / Humans Idioma: En Revista: J Histochem Cytochem Asunto de la revista: HISTOCITOQUIMICA Año: 2002 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Estados Unidos

Buscar en Google

Añadir a Mi BVS

Imprimir

XML

PubMed Links