Expression of a green fluorescence protein-carrier protein into mouse spermatozoa.
Biochem Biophys Res Commun
; 297(4): 841-6, 2002 Oct 04.
Article
en En
| MEDLINE
| ID: mdl-12359229
Intra-testicular inoculation of an adenoviral vector carrying the fusion gene Aequorea victoria green fluorescence protein/rat-liver glycogen synthase (GFP/LGS) resulted in the presence of GFP/GLS in spermatozoa from 7days to, at least, 16days after inoculation. The GFP/LGS was detected in the sperm heads after an "in vitro" fertilization procedure, either before or after the oocyte penetration. Our results indicate that spermatozoa carrying GFP/LGS protein conserved their fertilizing ability and were also detectable after oocyte penetration. This technique will allow to develop an easy system to follow the fate of mature sperm proteins.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Espermatozoides
/
Proteínas Luminiscentes
Límite:
Animals
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
2002
Tipo del documento:
Article
País de afiliación:
España
Pais de publicación:
Estados Unidos