Large scale sex typing of ostriches using DNA extracted from feathers.
BMC Biotechnol
; 2: 19, 2002 Oct 01.
Article
en En
| MEDLINE
| ID: mdl-12359044
BACKGROUND: Ostrich (Struthio camelus) breeds have been gaining increasing significance around the world. The large-scale sex determination of chicks is an important task in the development of these breeds. To date, two PCR-based methods have been established for ostrich sex typing, neither of them intended for large-scale analyses. Here, we report on a protocol adapted to carry out large-scale gender scoring using DNA obtained from chick feathers. RESULTS: The DNA was extracted using a fast and simple alkaline extraction protocol that provided sufficient template for an early diagnosis. Tests with several primer sets enabled us to determine the best internal control primers associated with the sex-specific primers, avoiding spurious bands. Using DNA extracted from a single bulb and the best set of primers, we applied this protocol to simultaneously sex-type 96 individuals accurately. CONCLUSION: We have established a fast, safe, accurate and inexpensive procedure for large-scale sex typing of ostriches using DNA extracted from feathers. This procedure is useful for the gender identification of chicks in the first days of nestling life.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Análisis para Determinación del Sexo
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Reacción en Cadena de la Polimerasa
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Struthioniformes
Tipo de estudio:
Evaluation_studies
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Guideline
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Prognostic_studies
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Screening_studies
Límite:
Animals
Idioma:
En
Revista:
BMC Biotechnol
Asunto de la revista:
BIOTECNOLOGIA
Año:
2002
Tipo del documento:
Article
País de afiliación:
Brasil
Pais de publicación:
Reino Unido