Variation in diosgenin levels among 10 accessions of fenugreek seeds produced in western Canada.
J Agric Food Chem
; 50(21): 5994-7, 2002 Oct 09.
Article
en En
| MEDLINE
| ID: mdl-12358471
A collection of 10 accessions of fenugreek (Trigonella foenum-graecum L.), an annual legume, was grown during two summers at three plot locations in western Canada to assess whether genetic (accession) and environmental factors (site and year of production) influenced levels of diosgenin, a steroidal sapogenin. The 60 harvested seed samples, each analyzed by single determinations on three subsamples of defatted and dried seed material, were hydrolyzed by a microscale procedure in water containing 2-propanol (70%) and sulfuric acid (1 M). The extracts were analyzed by capillary gas chromatography with 6-methyldiosgenin as internal standard. Diosgenin levels from mature seeds ranged from 0.28 to 0.92% (28-92 microg/10 mg). Analysis of variance on combined diosgenin levels from the three sites and two years revealed that accession, accession x year, and site x year effects were significant for diosgenin content, whereas site, year, and site x accession effects were not. Four accessions, CN 19062, CN 19067, CN 19070, and CN 19071, were identified with high levels of diosgenin on the basis of the 2-year data set. In these accessions, mean levels of diosgenin plus yamogenin from seven site years were estimated at 0.70, 0.98, 0.84, and 0.87%, respectively.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Semillas
/
Trigonella
/
Diosgenina
Tipo de estudio:
Prognostic_studies
País/Región como asunto:
America do norte
Idioma:
En
Revista:
J Agric Food Chem
Año:
2002
Tipo del documento:
Article
Pais de publicación:
Estados Unidos