Your browser doesn't support javascript.
loading
Therapeutic vaccination with vhs(-) herpes simplex virus reduces the severity of recurrent herpetic stromal keratitis in mice.
Keadle, Tammie L; Laycock, Keith A; Morris, Jessica L; Leib, David A; Morrison, Lynda A; Pepose, Jay S; Stuart, Patrick M.
Afiliación
  • Keadle TL; Washington University School of Medicine, Department of Ophthalmology and Visual Sciences, Box 8096, 660 South Euclid, St Louis, MO 63110, USA1.
  • Laycock KA; Washington University School of Medicine, Department of Ophthalmology and Visual Sciences, Box 8096, 660 South Euclid, St Louis, MO 63110, USA1.
  • Morris JL; Washington University School of Medicine, Department of Ophthalmology and Visual Sciences, Box 8096, 660 South Euclid, St Louis, MO 63110, USA1.
  • Leib DA; Washington University School of Medicine, Department of Ophthalmology and Visual Sciences, Box 8096, 660 South Euclid, St Louis, MO 63110, USA1.
  • Morrison LA; Washington University School of Medicine, Department of Ophthalmology and Visual Sciences, Box 8096, 660 South Euclid, St Louis, MO 63110, USA1.
  • Pepose JS; Washington University School of Medicine, Department of Ophthalmology and Visual Sciences, Box 8096, 660 South Euclid, St Louis, MO 63110, USA1.
  • Stuart PM; Washington University School of Medicine, Department of Ophthalmology and Visual Sciences, Box 8096, 660 South Euclid, St Louis, MO 63110, USA1.
J Gen Virol ; 83(Pt 10): 2361-2365, 2002 Oct.
Article en En | MEDLINE | ID: mdl-12237416
Virion host shutoff (vhs)-deficient herpes simplex virus (HSV) was tested as a therapeutic vaccine in a mouse model of UV light-induced recurrent herpetic stromal keratitis. Four weeks after primary corneal infection, mice were vaccinated intraperitoneally with vhs(-) vaccine or control. Four weeks after vaccination, the eyes of latently infected mice were UV-B irradiated to induce recurrent virus shedding and disease. Post-irradiation corneal opacity in latently infected, vhs(-)-vaccinated mice was significantly reduced compared to control-vaccinated mice (P=0.007 to 0.035). The incidence and duration of recurrent virus shedding were the same in both groups. Antibody titres were increased (P=0.05) and delayed type hypersensitive responses were unaffected by vhs(-) vaccination. Combined with studies using different vaccination timing and vhs(-) genotypes, these data suggest that deletion of vhs is a useful strategy in the development of a therapeutic HSV vaccine, and that temporal and genetic factors influence vaccination outcome.
Asunto(s)
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Virales / Queratitis Herpética / Herpesvirus Humano 1 / Opacidad de la Córnea / Vacunas contra el Virus del Herpes Simple Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J Gen Virol Año: 2002 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Virales / Queratitis Herpética / Herpesvirus Humano 1 / Opacidad de la Córnea / Vacunas contra el Virus del Herpes Simple Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J Gen Virol Año: 2002 Tipo del documento: Article Pais de publicación: Reino Unido