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Depolarization-Activated K+ Channel in Chara Droplets.
Pottosin, I. I.; Andjus, P. R..
Afiliación
  • Pottosin II; Institute of General and Physical Chemistry, P.O. Box 551, 11000 Belgrade, Yugoslavia.
Plant Physiol ; 106(1): 313-319, 1994 Sep.
Article en En | MEDLINE | ID: mdl-12232330
A novel potassium channel was characterized in the droplet membrane of Chara gymnophylla. This channel has a conductance of about 90 pS (in symmetrical 0.15 M KCl), which is lower compared to the 170-pS K+ channel predominant in this preparation. In contrast to the large conductance K+ channel, the novel channel opened with a delay at depolarization and closed at hyperpolarization and did not require cytosolic Ca2+ for its opening. It also showed comparatively weak selectivity for K+ over other monovalent cations, although its cation to anion selectivity was high. Externally or internally applied Cs+ blocked the channel in a voltage-dependent manner, similarly to the 170-pS channel. The sensitivity of the 90-pS channel to external tetraethylammonium chloride (half-blocking concentration approximately 1.5 mM) was 20-fold higher compared to the large conductance channel. With respect to its voltage-gating kinetics, the 90-pS channel was identified as a "slow delayed rectifier."

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Plant Physiol Año: 1994 Tipo del documento: Article País de afiliación: Yugoslavia Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Plant Physiol Año: 1994 Tipo del documento: Article País de afiliación: Yugoslavia Pais de publicación: Estados Unidos