Restriction endonuclease and monoclonal antibody analysis of Brazilian isolates of bovine herpesviruses types 1 and 5.
Vet Microbiol
; 88(4): 315-24, 2002 Sep 24.
Article
en En
| MEDLINE
| ID: mdl-12220807
Twelve Brazilian isolates and three reference strains of bovine herpesviruses (BHVs) were subjected to restriction endonuclease analysis (REA) and monoclonal antibody (MAb) analysis. Viral DNA was cleaved with BamHI, BstEII, EcoRI, HindIII and PstI. The monoclonal antibody panel allowed the differentiation between types 1 and 5 viruses, while REA with BstEII and HindIII showed the distinction between BHV-1 and -5 subtypes. Typical 1.1 and 1.2a patterns were observed with two isolates from respiratory disease. An isolate from semen of a clinically healthy bull displayed 1.2b profile, whereas another displayed a clear 5a pattern, which was never reported before. Seven out of nine Brazilian type 5 (BHV-5) isolates displayed REA patterns similar to the Australian BHV-5 strain N569 (BHV-5a), and differing from the Argentinean A663 strain (BHV-5b) virus. Another two BHV-5 isolates, which displayed an unusual MAb pattern of reactivity, showed a BstEII profile different from both reference strains of BHV-5. These two viruses were considered BHV-5 "non-a/non-b" subtype.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Enfermedades de los Bovinos
/
Infecciones por Herpesviridae
/
Herpesvirus Bovino 1
/
Encefalitis Viral
/
Herpesvirus Bovino 5
/
Meningoencefalitis
Límite:
Animals
País/Región como asunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Vet Microbiol
Año:
2002
Tipo del documento:
Article
País de afiliación:
Brasil
Pais de publicación:
Países Bajos