A cell type-specific allele of the POU gene Oct-6 reveals Schwann cell autonomous function in nerve development and regeneration.
EMBO J
; 21(17): 4612-20, 2002 Sep 02.
Article
en En
| MEDLINE
| ID: mdl-12198163
While an important role for the POU domain transcription factor Oct-6 in the developing peripheral nerve has been well established, studies into its exact role in nerve development and regeneration have been hampered by the high mortality rate of newborn Oct-6 mutant animals. In this study we have generated a Schwann cell-specific Oct-6 allele through deletion of the Schwann cell-specific enhancer element (SCE) in the Oct-6 locus. Analysis of mice homozygous for this allele (deltaSCE allele) reveals that rate-limiting levels of Oct-6 in Schwann cells are dependent on the SCE and that this element does not contribute to Oct-6 regulation in other cell types. We demonstrate a Schwann cell autonomous function for Oct-6 during nerve development as well as in regenerating nerve. Additionally, we show that Krox-20, an important regulatory target of Oct-6 in Schwann cells, is activated, with delayed kinetics, through an Oct-6-independent mechanism in these mice.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Nervios Periféricos
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Células de Schwann
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Factores de Transcripción
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Elementos de Facilitación Genéticos
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Regeneración Nerviosa
Límite:
Animals
Idioma:
En
Revista:
EMBO J
Año:
2002
Tipo del documento:
Article
País de afiliación:
Países Bajos
Pais de publicación:
Reino Unido