Solid-state NMR relaxation studies of Australian spider silks.
Biopolymers
; 61(4): 287-97, 2001.
Article
en En
| MEDLINE
| ID: mdl-12115143
Solid-state NMR techniques were used to study two different types of spider silk from two Australian orb-web spider species, Nephila edulis and Argiope keyserlingi. A comparison of (13)C-T(1) and (1)H-T(1rho) solid-state NMR relaxation data of the Ala Calpha, Ala Cbeta, Gly Calpha, and carbonyl resonances revealed subtle differences between dragline and cocoon silk. (13)C-T(1rho) and (1)H-T(1) relaxation experiments showed significant differences between silks of the two species with possible structural variations. Comparison of our data to previous (13)C-T(1) relaxation studies of silk from Nephila clavipes (A. Simmons et al., Macromolecules, 1994, Vol. 27, pp. 5235-5237) also supports the finding that differences in molecular mobility of dragline silk exist between species. Interspecies differences in silk structure may be due to different functional properties. Relaxation studies performed on wet (supercontracted) and dry silks showed that the degree of hydration affects relaxation properties, and hence changes in molecular mobility are correlated with functional properties of silk.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Arañas
/
Proteínas de Insectos
Límite:
Animals
País/Región como asunto:
Oceania
Idioma:
En
Revista:
Biopolymers
Año:
2001
Tipo del documento:
Article
Pais de publicación:
Estados Unidos