Myenteric neurons and interstitial cells of Cajal of mouse colon express several nitric oxide synthase isoforms.
Neurosci Lett
; 326(3): 191-5, 2002 Jul 05.
Article
en En
| MEDLINE
| ID: mdl-12095654
Information on equipment and subcellular distribution of nitric oxide synthase (NOS) isoforms in myenteric neurons and pacemaker cells (ICC) might help to identify nitric oxide (NO) pathway(s) acting on gastrointestinal motility. In sections of mouse colon labelled with neuronal (n)NOS, endothelial (e)NOS and inducible (i)NOS antibodies, all myenteric neurons co-expressed eNOS and iNOS and a subpopulation of them co-expressed nNOS. ICC co-expressed nNOS and eNOS. In the neurons, nNOS-labeling was intracytoplasmatic, in the ICC at cell periphery. In both cell types, eNOS-labeling was on intracytoplasmatic granules, likely mitochondria. In conclusion, myenteric neurons and ICC co-express several NOS isoforms with specific subcellular distribution. Different nNOS splice variants are presumably present: intracytoplasmatic nNOSbeta and nNOSalpha producing neurogenic NO, plasma membrane-bound nNOSalpha producing ICCgenic NO. eNOS might be implicated in mitochondrial respiration and, in ICC, also in pacemaker activity. Neurons express iNOS also in basal condition.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Óxido Nítrico Sintasa
/
Neuronas
Límite:
Animals
Idioma:
En
Revista:
Neurosci Lett
Año:
2002
Tipo del documento:
Article
País de afiliación:
Italia
Pais de publicación:
Irlanda