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Cardioprotective effects of 9-hydroxyellipticine on ischemia and reperfusion in isolated rat heart.
Saeki, Kazuhiko; Obi, Ichiro; Ogiku, Noriko; Shigekawa, Munekazu; Imagawa, Toshiaki; Matsumoto, Takeshi.
Afiliación
  • Saeki K; Discovery Research Laboratory, Tanabe Seiyaku Co, Toda-shi, Saitama, Japan. kazu@tanabe.co.jp
Jpn J Pharmacol ; 89(1): 21-8, 2002 May.
Article en En | MEDLINE | ID: mdl-12083739
We determined the effect of 9-hydroxyellipticine (9HE) on ryanodine receptor (RyR) and cardiac function after global ischemia in isolated rat hearts. The binding of [3H]-ryanodine in rabbit cardiac sarcoplasmic reticulum was displaced by 9HE in a biphasic manner corresponding to the two sites model with IC50 values of 6.1 microM and 55 mM. The increase of the intracellular Ca2+ concentration induced by caffeine in CHO cells expressing cardiac-type RyR was suppressed by 9HE in a concentration-dependent manner. Pretreatment of the heart with 9HE decreased the total duration of reperfusion-induced ventricular fibrillation (VF) and delayed the onset of VF. There was also a significant recovery of contractile force of ischemic hearts following 9HE. Unlike nifedipine, an L-type Ca2+-channel blocker, 9HE did not suppress the contraction of rat papillary muscles. Thus, 9HE exerts the cardioprotective effects against ischemia /reperfusion injury without changing hemodynamic indices.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Arritmias Cardíacas / Daño por Reperfusión Miocárdica / Isquemia Miocárdica / Elipticinas / Antiarrítmicos Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Jpn J Pharmacol Año: 2002 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Japón
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Arritmias Cardíacas / Daño por Reperfusión Miocárdica / Isquemia Miocárdica / Elipticinas / Antiarrítmicos Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Jpn J Pharmacol Año: 2002 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Japón