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Liver necrosis induced by acute intraperitoneal ethanol administration in aged rats.
Giavarotti, Leandro; D'Almeida, Vania; Giavarotti, Karin A S; Azzalis, Ligia A; Rodrigues, Luciano; Cravero, Amerys A M; Videla, Luis A; Koch, Osvaldo R; Junqueira, Virginia B C.
Afiliación
  • Giavarotti L; Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Brazil. junqueira-geriat@pesquisa.epm.br
Free Radic Res ; 36(3): 269-75, 2002 Mar.
Article en En | MEDLINE | ID: mdl-12071345
It is generally agreed that the deleterious pathophysiological effects of ethanol are caused, at least partially by an increase in free radical production. However, little attention has been directed to the effects of ethanol upon elderly organisms. Male Wistar rats at ages 3, 6, 12, 18 and 24 months were treated either with a single i.p. dose of 35% ethanol (v/v) at 3 g ethanol/kg body weight or an isovolumetric amount of 0.9% saline solution. We then assessed the plasma levels of transaminases and hepatic levels of oxidative stress-related parameters, followed by liver histological evaluation. The younger rats (3 months old) were not affected by the treatment with ethanol with respect to any of the studied parameters except for a lowering of total hepatic GSH and an increase in hepatic thiobarbituric acid reactants (TBARS) formation, while animals older than 3 months were increasingly more affected by the treatment. Acute ethanol treatment elicited the similar responses to those in the 3 months-old group, plus a decrease in the hepatic and plasma levels of beta-carotene and the plasma level of alpha-tocopherol, as well as an increase in the activity of plasma transaminases. In the 12,18 and 24 months old groups, there was increasing liver necrosis. These findings suggest that liver damage induced by acute ethanol administration in elderly rats may involve a lack of antioxidants.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hexaclorociclohexano / Envejecimiento / Oxidantes / Etanol / Hígado / Cirrosis Hepática Alcohólica Límite: Animals Idioma: En Revista: Free Radic Res Asunto de la revista: BIOQUIMICA Año: 2002 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hexaclorociclohexano / Envejecimiento / Oxidantes / Etanol / Hígado / Cirrosis Hepática Alcohólica Límite: Animals Idioma: En Revista: Free Radic Res Asunto de la revista: BIOQUIMICA Año: 2002 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido