Swift and definite serotyping for isolated Listeria monocytogenes strains.
New Microbiol
; 25(2): 165-71, 2002 Apr.
Article
en En
| MEDLINE
| ID: mdl-12019722
The serotype is most important for molecular epidemiological analysis of Listeria monocytogenes (L.m.) contaminating marketed meats. An improvement on the traditional method was thus attempted in the present study because of the requirement of swift and definite serotyping. In the determination of O-antigen, definite judgement was allowed by an immediate cooling at 80 degrees C after autoclaving the bacteria. In the determination of H-antigen, use of a culture plate without Craigie's tube yielded the active bacteria only by single culture. The stable and clear agglutination in many samples was also obtained with a microplate using less antiserum. The availability was confirmed with 123 strains and the serovar 1/2b was dominant in the Japanese strains.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Serotipificación
/
Listeria monocytogenes
Límite:
Humans
Idioma:
En
Revista:
New Microbiol
Asunto de la revista:
MICROBIOLOGIA
Año:
2002
Tipo del documento:
Article
País de afiliación:
Japón
Pais de publicación:
Italia