Induction of 8-hydroxy-2'-deoxyguanosine by cobalt(II) and hydrogen peroxide in vitro.
J Toxicol Environ Health A
; 65(9): 665-76, 2002 May 10.
Article
en En
| MEDLINE
| ID: mdl-11996407
In order to test the effects of cobalt on oxidative DNA damage, we measured the formation of 8-hydroxy-2'-deoxyguanosine (8-OHdG) in the presence of cobalt in calf thymus DNA and in DNA of human diploid fibroblasts. Treatment of calf thymus DNA with Co(II) at 500 microM hydrogen peroxide resulted in a dose-dependent increase in 8-OHdG, which culminated at 25 microM Co(II) (62.6 modified/10(5) dG) and declined at higher Co(II) concentrations [9.6 modified/10(5) dG at 250 microM Co(II)]. Incubation of calf thymus DNA with Co(II) alone did not cause an increase in 8-OHdG. Treatment of calf thymus DNA with H2O2, (0.25-2 mM) caused only a slight generation of 8-OHdG (2.7/10(5) dG, at 2 mM H2O2). Exposure of human diploid fibroblasts to Co(II) at 5-250 microM did not result in an increase in 8-OHdG in a dose-dependent manner, although treated cells showed significantly higher 8-OHdG levels than untreated controls (2.026 +/- 0.695 vs. 1.395 +/- 0.433 8-OHdG/10(5) dG) at all concentrations. Our data indicate that in the presence of H2O2, Co(II) stimulates the in vitro formation of 8-OHdG.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Oxidantes
/
Cobalto
/
Desoxiguanosina
/
Peróxido de Hidrógeno
Límite:
Animals
/
Humans
Idioma:
En
Revista:
J Toxicol Environ Health A
Asunto de la revista:
SAUDE AMBIENTAL
/
TOXICOLOGIA
Año:
2002
Tipo del documento:
Article
País de afiliación:
Austria
Pais de publicación:
Reino Unido