Influence of thalidomide on Bcl2 expression and proangiogenic cytokine levels in short-term culture of peripheral blood and bone marrow mononuclear cells of multiple myeloma patients.
Pol J Pharmacol
; 53(6): 709-13, 2001.
Article
en En
| MEDLINE
| ID: mdl-11985351
Supernatants from short-term culture of peripheral blood and bone marrow mononuclear cells obtained from 22 multiple myeloma patients were used to measure the concentration of TNF-alpha, HGF, IL-6 and its soluble receptor (sIL-6R), VEGF and bFGF. Cells were cultured with or without thalidomide (THAL). We observed statistically significant decrease in TNF-alpha, HGF, IL-6, sIL-6R in supernatants from THAL cultures compared to cells cultured without THAL. Flow cytometry technique was applied to study the Bcl2 expression on CD 4, CD 8 and CD 138 positive cells. The statistically significant decrease in Bcl2 expression on myeloma cells (CD 138+) was observed both in PB and BM cultures. THAL could inhibit the plasma cell growth both by diminishing proangiogenic cytokines production and enhancing myeloma cell apoptosis.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Talidomida
/
Células de la Médula Ósea
/
Leucocitos Mononucleares
/
Citocinas
/
Proteínas Proto-Oncogénicas c-bcl-2
/
Inhibidores de la Angiogénesis
/
Mieloma Múltiple
Límite:
Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
Pol J Pharmacol
Asunto de la revista:
FARMACOLOGIA
Año:
2001
Tipo del documento:
Article
País de afiliación:
Polonia
Pais de publicación:
Polonia