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Tefluthrin modulates a novel anionic background conductance (I(AB)) in guinea-pig ventricular myocytes.
Borg, John J; Hancox, Jules C; Spencer, C Ian; Kozlowski, Roland Z.
Afiliación
  • Borg JJ; Department of Pharmacology, University of Bristol, Bristol, BS8 1TD, United Kingdom.
Biochem Biophys Res Commun ; 292(1): 208-15, 2002 Mar 22.
Article en En | MEDLINE | ID: mdl-11890694
This report describes for the first time a novel anionic background current (I(AB)) identified in guinea-pig isolated ventricular myocytes. It also shows that I(AB) has both novel and differential pharmacology from other (cardiac) chloride currents. Using the whole-cell patch-clamp technique and external anion substitution, I(AB) was found to be outwardly rectifying and highly permeable to NO(-)(3), with a relative permeability sequence of NO(-)(3) > I(-) > Cl(-). I(AB) was not blocked by 50 microM DIDS, by hypertonic external solution, or by the nonselective protein kinase inhibitor H7-DHC. Exposure to the pyrethroid agent tefluthrin (10 microM) increased the current density of I(AB) significantly at positive voltages (P < 0.05), but had no significant effect on other cardiac chloride currents. We conclude that I(AB) possesses a distinct pharmacology and does not fall into the three major classes of cardiac chloride conductance commonly reported.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Piretrinas / Corazón / Insecticidas Límite: Animals Idioma: En Revista: Biochem Biophys Res Commun Año: 2002 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Piretrinas / Corazón / Insecticidas Límite: Animals Idioma: En Revista: Biochem Biophys Res Commun Año: 2002 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Estados Unidos