[Detection and proliferation of hemorrhagic fever virus in chigger mites].

Zhang, Y; Zhu, J; Tang, J; Li, X; Wu, G

Zhang, Y; Zhu, J; Tang, J; Li, X; Wu, G.

Afiliación

Zhang Y; Institute for Military Medicine, Nanjing Command of PLA, Nanjing 210002.

Zhonghua Yu Fang Yi Xue Za Zhi ; 33(2): 98-100, 1999 Mar.

Article en Zh | MEDLINE | ID: mdl-11864463

RESUMEN

OBJECTIVE: To study the impact of Leptotromhidium scutellaris as a vector in transmission of hemorrhagic fever. METHODS: Reverse transcription-polymerase chain reaction (PCR) and in situ hybridization technique were used to detect RNA of hemorrhagic fever with renal syndrome virus (HFRSV). TCID(50)/ml of HFRSV were titrated for larva and nymph of chigger mites periodically. RESULTS: RNA of HFRSV was detected in the tissues of chigger mites, such as ovary cells, etc. by PCR and in situ hybridization. Titration of TCID(50)/ml showed that HFRSV could be transmitted transstadially and proliferated in chigger mites. CONCLUSION: It provides direct evidence at molecular level for the role of chigger mite as a vector in transmission of HFRSV with theoretical and practical importance in prevention of HFRS.

Asunto(s)

Virus Hantaan/aislamiento & purificación; Fiebre Hemorrágica con Síndrome Renal/transmisión; Trombiculidae/virología; Animales; Virus Hantaan/crecimiento & desarrollo; Fiebre Hemorrágica con Síndrome Renal/prevención & control; Insectos Vectores/virología; ARN Viral/análisis

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trombiculidae / Virus Hantaan / Fiebre Hemorrágica con Síndrome Renal Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: Zh Revista: Zhonghua Yu Fang Yi Xue Za Zhi Año: 1999 Tipo del documento: Article Pais de publicación: China

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