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A neutrophil multitarget functional bioassay to detect anti-inflammatory natural products.
Johansson, Senia; Göransson, Ulf; Luijendijk, Teus; Backlund, Anders; Claeson, Per; Bohlin, Lars.
Afiliación
  • Johansson S; Division of Pharmacognosy, Department of Medicinal Chemistry, Biomedical Centre, Uppsala University, PO Box 574, S-751 23 Uppsala, Sweden.
J Nat Prod ; 65(1): 32-41, 2002 Jan.
Article en En | MEDLINE | ID: mdl-11809061
A multitarget functional bioassay was optimized as a method for detecting substances interacting with the inflammatory process of activated neutrophil granulocytes, mainly to release elastase detected by p-nitroanilide (pNA) formation. Using this bioassay, 100 fractionated extracts of 96 plants were screened, with results presented in a manner that links recorded biological activity to phylogenetic information. The plants were selected to represent a major part of the angiosperms, with emphasis on medicinal plants, Swedish anti-inflammatory plants, and plants known to contain peptides. Of the tested extracts, 41% inhibited pNA formation more than 60%, and 3% stimulated formation. The extract of Digitalis purpurea enhanced pNA formation, and digitoxin, the active compound, was isolated and identified. Plant extracts that exhibited potent nonselective inhibition (>80% inhibition) were evaluated further for direct inhibition of isolated elastase and trypsin enzyme. The inhibitory effect of most tested extracts on the isolated enzyme elastase was similar to that of PAF- and fMLP-induced pNA formation. Compared to trypsin, inhibition of elastase by extracts of Rubus idaeus and Tabernaemontana dichotoma was significantly higher (80% and 99%, respectively). Inhibition of trypsin by the extract of Reseda luteola was high (97%). Orders such as Lamiales and Brassicales were shown to include a comparably high proportion of plants with inhibitory extracts.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plantas Medicinales / Bioensayo / Productos Biológicos / Activación Neutrófila / Elastasa de Leucocito / Magnoliopsida / Antiinflamatorios / Neutrófilos Tipo de estudio: Prognostic_studies País/Región como asunto: Europa Idioma: En Revista: J Nat Prod Año: 2002 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Estados Unidos
Buscar en Google
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plantas Medicinales / Bioensayo / Productos Biológicos / Activación Neutrófila / Elastasa de Leucocito / Magnoliopsida / Antiinflamatorios / Neutrófilos Tipo de estudio: Prognostic_studies País/Región como asunto: Europa Idioma: En Revista: J Nat Prod Año: 2002 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Estados Unidos