A modified cup scrub method for assessing the antibacterial substantivity of personal cleansing products.
J Cosmet Sci
; 52(6): 369-75, 2001.
Article
en En
| MEDLINE
| ID: mdl-11773954
An improved in vivo method for evaluating the antibacterial substantivity or residual effectiveness of bar soaps and other personal cleansing products is presented. The effectiveness of an antibacterial bar soap containing 1.5% 3, 4, 4'-trichlorocarbanilide (TCC) versus its soap vehicle was evaluated under simulated conditions considered optimal for bacterial growth, proliferation, and possible infection. A washout period to clear the skin of any antimicrobial agents previously used was followed by a treatment period in which the subjects washed one of their forearms with the antibacterial soap and the other forearm with the soap vehicle. Either immediately or 24 hours following the final wash, three test sites on both forearms were inoculated with S. aureus and occluded with Hill Top Chamber patches. At intervals of 30 minutes, two hours and five hours, the patches were removed. The bacteria on the skin were harvested using the Williamson-Kligman scrub technique (1,2) to determine the number of surviving CFUs at each time period. The method successfully demonstrated that sufficient TCC had remained on the skin for 24 hours after the final wash to effectively inhibit the growth of S. aureuso on the skin for as long as five hours after inoculation.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Staphylococcus aureus
/
Pruebas de Sensibilidad Microbiana
/
Desinfección de las Manos
/
Higiene
/
Cosméticos
/
Antibacterianos
Límite:
Adolescent
/
Adult
/
Aged
/
Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
J Cosmet Sci
Asunto de la revista:
QUIMICA CLINICA
Año:
2001
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Estados Unidos