B9-serine residue is crucial for insulin actions in glucose metabolism.
Acta Pharmacol Sin
; 22(10): 939-43, 2001 Oct.
Article
en En
| MEDLINE
| ID: mdl-11749779
AIM: To explore the importance of B9 and B10 amino acid residues in the insulin molecule. METHODS: The [B9Glu, B10Asp] insulin (E,D-insulin) receptor binding activity, glucose upta ke activity, and lipogenesis activity were measured in isolated adipocytes. The translocation of glucose transporter 4 (Glut4) and the phosphorylation of insulin receptor (IR) stimulated by E, D-insulin were determined by Western blotting. RESULTS: Compared with native insulin, the receptor binding activity of E,D-insulin was 31 %; the stimulating activity of E,D-insulin in glucose transport and lipogenesis were 45 % and 40 % respectively; the stimulations of Glut4 translocation and insulin receptor autophosphorylation of E,D-insulin were about 58 % and 46 % respectively. CONCLUSION: B9-serine residue is crucial for insulin actions in glucose metabolism.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Serina
/
Receptor de Insulina
/
Glucosa
/
Insulina
Límite:
Animals
Idioma:
En
Revista:
Acta Pharmacol Sin
Asunto de la revista:
FARMACOLOGIA
Año:
2001
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
Estados Unidos