Differentiation of resting human peripheral blood gamma delta T cells toward Th1- or Th2-phenotype.
Cell Immunol
; 212(2): 110-7, 2001 Sep 15.
Article
en En
| MEDLINE
| ID: mdl-11748927
Depending on the microenvironment, murine gamma delta T cells differentiate into either Th1 (IFN-gamma-producing) or Th2 (IL-4-producing) cells. It is unclear, however, whether circulating human peripheral blood gamma delta T cells can be driven into Th1 or Th2 cells by modulation of the priming cytokine milieu. In this study, peripheral blood gamma delta T cells were stimulated by phosphoantigen (isopentenyl pyrophosphate) or Daudi lymphoma cells in the presence of Th1-priming (rIL-12, anti-IL-4 Ab) or Th2-priming (rIL-4, anti-IL-12 Ab) conditions. Single-cell analysis of cytokine secretion (IFN-gamma and IL-4) was performed by flow cytometry after 18 h and after restimulation of expanded gamma delta T cells. The early activation of resting gamma delta T cells was characterized by the induction of IFN-gamma. Priming under Th1 conditions induced a Th1 profile characterized by increased secretion of IFN-gamma and TNF-alpha, while Th2 conditions caused increased production of IL-4 (Th2 profile) by the gamma delta T cells. These results indicate that the major subset of human gamma delta T cells can be polarized into either Th1 or Th2 cytokine pattern depending on the cytokine milieu in which contact with antigen occurs.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Subgrupos de Linfocitos T
/
Receptores de Antígenos de Linfocitos T gamma-delta
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Células Th2
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Células TH1
/
Hemiterpenos
Límite:
Humans
Idioma:
En
Revista:
Cell Immunol
Año:
2001
Tipo del documento:
Article
País de afiliación:
Alemania
Pais de publicación:
Países Bajos