Activity of human Delta5 and Delta6 desaturases on multiple n-3 and n-6 polyunsaturated fatty acids.

de Antueno, R J; Knickle, L C; Smith, H; Elliot, M L; Allen, S J; Nwaka, S; Winther, M D

de Antueno, R J; Knickle, L C; Smith, H; Elliot, M L; Allen, S J; Nwaka, S; Winther, M D.

Afiliación

de Antueno RJ; QuantaNova Canada Ltd., P.O. Box 818, Kentville, NS, B4N 4H8, Canada. r.deantueno@mailexcite.com

FEBS Lett ; 509(1): 77-80, 2001 Nov 30.

Article en En | MEDLINE | ID: mdl-11734209

RESUMEN

Yeast co-expressing human elongase and desaturase genes were used to investigate whether the same desaturase gene encodes an enzyme able to desaturate n-3 and n-6 fatty acids with the same or different carbon chain length. The results clearly demonstrated that a single human Delta5 desaturase is active on 20:3n-6 and 20:4n-3. Endogenous Delta6 desaturase substrates were generated by providing to the yeast radiolabelled 20:4n-6 or 20:5n-3 which, through two sequential elongations, produced 24:4n-6 and 24:5n-3, respectively. Overall, our data suggest that a single human Delta6 desaturase is active on 18:2n-6, 18:3n-3, 24:4n-6 and 24:5n-3.

Asunto(s)

Ácido Graso Desaturasas/metabolismo; Ácidos Grasos Insaturados/metabolismo; Cromatografía Líquida de Alta Presión; Clonación Molecular; ADN Complementario/metabolismo; delta-5 Desaturasa de Ácido Graso; Humanos; Cinética; Linoleoil-CoA Desaturasa; Metabolismo de los Lípidos; Modelos Químicos; Saccharomyces cerevisiae/metabolismo; Factores de Tiempo

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ácido Graso Desaturasas / Ácidos Grasos Insaturados Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: FEBS Lett Año: 2001 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Reino Unido

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