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Presence of genetic alterations in microdissected stroma of human colon and breast cancers.
Wernert, N; Löcherbach, C; Wellmann, A; Behrens, P; Hügel, A.
Afiliación
  • Wernert N; Institute of Pathology, University of Bonn, Germany. wernert@meb.uni-bonn.de
Anticancer Res ; 21(4A): 2259-64, 2001.
Article en En | MEDLINE | ID: mdl-11724280
BACKGROUND: Human carcinomas not only consist of neoplastic epithelial cells but also of tumor stroma, which may play an important role in tumor-progression. Whereas the tumor surrounding stroma is generally believed to represent a reactive component induced by tumor cell derived factors, a contribution of neoplastic cells to stroma formation via epithelium-mesenchyme transition during tumor invasion has become a novel concept in recent years. MATERIALS, METHODS AND RESULTS: We here show, by laser-assisted microdissection, that frequent genetic alterations in non-hereditary invasive human colon and breast cancers (loss of heterozygosity and TP53 mutations) occur not only in the neoplastic epithelial cells, but also in the adjacent fibroblastic stroma and that both components can share clonal features. CONCLUSION: Tumor cell-mesenchyme transitions are among the possible explanations for these findings and could actually occur during tumor invasion in vivo.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Mama / Neoplasias del Colon / Carcinoma Ductal de Mama Límite: Humans Idioma: En Revista: Anticancer Res Año: 2001 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Grecia
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Mama / Neoplasias del Colon / Carcinoma Ductal de Mama Límite: Humans Idioma: En Revista: Anticancer Res Año: 2001 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Grecia