Crystallization and preliminary X-ray analysis of the periplasmic nitrate reductase (NapA-NapB complex) from Rhodobacter sphaeroides f. sp. denitrificans.
Acta Crystallogr D Biol Crystallogr
; 57(Pt 12): 1900-2, 2001 Dec.
Article
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| MEDLINE
| ID: mdl-11717511
The periplasmic nitrate reductase of Rhodobacter sphaeroides f. sp. denitrificans is a heterodimer responsible for the first step of reduction in the denitrification process by the conversion of nitrate to nitrite. It consists of a 91 kDa molybdenum-containing catalytic subunit (NapA) and a 17 kDa dihaem cytochrome c (NapB). Crystals of the NapA-NapB complex were obtained by the vapour-diffusion method using ammonium sulfate as precipitant. They belong to the P6(1)22 space group, with unit-cell parameters a = b = 151.9, c = 255.8 A, and contain a single complex in the asymmetric unit. A complete native data set was collected at a synchrotron source to 3.1 A resolution.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Rhodobacter sphaeroides
/
Nitrato Reductasas
Idioma:
En
Revista:
Acta Crystallogr D Biol Crystallogr
Año:
2001
Tipo del documento:
Article
País de afiliación:
Francia
Pais de publicación:
Estados Unidos