Calculated background elimination in quantifying nitric-oxide synthase enzyme activity.

Stonek, F; Tschugguel, W; Huber, J C; Schneeberger, C

Stonek, F; Tschugguel, W; Huber, J C; Schneeberger, C.

Afiliación

Stonek F; Department of Gynecology and Obstetrics, Division of Gynecological Endocrinology and Reproductive Medicine, University Hospital Vienna, Waehringer Guertel 18-20 EBO5Q, A-1090, Vienna, Austria. felix.stonek@akh-wien.ac.at

J Biochem Biophys Methods ; 50(1): 29-32, 2001 Dec 04.

Article en En | MEDLINE | ID: mdl-11714509

RESUMEN

Measuring nitric-oxide synthase (NOS) activity by monitoring the conversion of L-arginine to L-citrulline is currently the standard assay for NOS activity. We describe a simple method of quantifying low values of NOS activity by removing the background mathematically. When performing NOS activity studies in samples with low protein amount (< 25 microg/microl), we encountered the problem of sample values that can hardly be differentiated from blank values probably originating from radioactive-labeled arginine in the final eluate. Our method determines mathematically these background values and may be an improvement of the citrulline assay.

Asunto(s)

Bioquímica/métodos; Óxido Nítrico Sintasa/biosíntesis; Óxido Nítrico Sintasa/química; Arginina/química; Citrulina/química; Humanos; Modelos Teóricos; Temperatura

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bioquímica / Óxido Nítrico Sintasa Límite: Humans Idioma: En Revista: J Biochem Biophys Methods Año: 2001 Tipo del documento: Article País de afiliación: Austria Pais de publicación: Países Bajos

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