Immunofluorescence assay reactivity patterns of serum samples presenting indeterminate Western blot results for antibodies to HIV-1 and HTLV-I/II in Cordoba, Argentina.
Rev Inst Med Trop Sao Paulo
; 43(5): 277-82, 2001.
Article
en En
| MEDLINE
| ID: mdl-11696851
Serum samples (n: 110) from blood donors and high risk individuals from Cordoba, Argentina with indeterminate HIV-1 and HTLV-I/II Wb profiles were studied for specific antibodies to HTLV-I/II and HIV-1 by indirect immunofluorescence assay (IFA) and for the presence or absence of HIV-1 and HTLV-I/II specific bands by Wb. This study was carried out in order to characterize their putative reactions with HIV-1 and HTLV-I/II proteins and to resolve the retrovirus infection status of these individuals. Results indicated that blood donors sera displaying indeterminate HIV-1 or HTLV-I/II Wb patterns were not immunoreactive to HTLV-I/II and HIV-1 on IFA. However, a high rate of indeterminate HIV-1 and HTLV-I/II Wb samples from high risk individuals had positive HTLV-I/II and HIV-1 IFA results respectively. Our study supports the growing evidence that HTLV-HIV indeterminate seroreactivity in low risk population is due to a cross reaction against nonviral antigens, and in high risk populations the indeterminate samples show serological cross-recognition between HIV-1 proteins and HTLV-I/II proteins on Wb. These results point out the necessity to investigate the HTLV-I/II reactivity in indeterminate HIV-1 samples and vice versa in order to confirm the diagnosis. Finally, this study shows the potential usefulness of IFA in elucidating the status of HIV-1 and HTLV-I/II infection of individuals with indeterminate Wb profiles, thus enabling resolution of retrovirus infection status.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Anticuerpos Antideltaretrovirus
/
Western Blotting
/
Infecciones por Retroviridae
/
Técnica del Anticuerpo Fluorescente Indirecta
Tipo de estudio:
Evaluation_studies
Límite:
Female
/
Humans
/
Male
País/Región como asunto:
America do sul
/
Argentina
Idioma:
En
Revista:
Rev Inst Med Trop Sao Paulo
Año:
2001
Tipo del documento:
Article
País de afiliación:
Argentina
Pais de publicación:
Brasil