Bisulfite genomic sequencing of microdissected cells.
Nucleic Acids Res
; 29(21): E106-6, 2001 Nov 01.
Article
en En
| MEDLINE
| ID: mdl-11691943
Mapping of methylation patterns in CpG islands has become an important tool for understanding tissue-specific gene expression in both normal and pathological situations. However, the inherent cellular heterogeneity of any given tissues can affect the outcome and interpretation of molecular studies. In order to analyse genomic DNA methylation on a pure cell population from tissue sample, we have developed a simple technique of single-cell microdissection from cryostat sections which can be combined with bisulfite-mediated sequencing of 5-methylcytosine. We report here our results on the methylation status of the androgen receptor gene studied by bisulfite genomic sequencing on purified cells isolated from human testis.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Sulfitos
/
Receptores Androgénicos
/
Genoma
/
Análisis de Secuencia de ADN
/
Metilación de ADN
Límite:
Humans
/
Male
Idioma:
En
Revista:
Nucleic Acids Res
Año:
2001
Tipo del documento:
Article
País de afiliación:
Francia
Pais de publicación:
Reino Unido