Selection analyses of insertional mutants using subgenic-resolution arrays.
Nat Biotechnol
; 19(11): 1060-5, 2001 Nov.
Article
en En
| MEDLINE
| ID: mdl-11689852
We describe a method of genome-wide analysis of quantitative growth phenotypes using insertional mutagenesis and DNA microarrays. We applied the method to assess the fitness contributions of Escherichia coli gene domains under specific growth conditions. A transposon library was subjected to competitive growth selection in Luria-Bertani (LB) and in glucose minimal media. Transposon-containing genomic DNA fragments from the selected libraries were compared with the initial unselected transposon insertion library on DNA microarrays to identify insertions that affect fitness. Genes involved in the biosynthesis of nutrients not provided in the growth medium were found to be significantly enriched in the set of genes containing negatively selected insertions. The data also identify fitness contributions of several uncharacterized genes, including putative transcriptional regulators and enzymes. The applicability of this high-resolution array selection in other species is discussed.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Mutagénesis Insercional
/
Huella de ADN
/
Análisis de Secuencia por Matrices de Oligonucleótidos
/
Escherichia coli
Tipo de estudio:
Evaluation_studies
Idioma:
En
Revista:
Nat Biotechnol
Asunto de la revista:
BIOTECNOLOGIA
Año:
2001
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Estados Unidos