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Human aryl hydrocarbon receptor polymorphisms that result in loss of CYP1A1 induction.
Wong, J M; Okey, A B; Harper, P A.
Afiliación
  • Wong JM; Department of Pharmacology, University of Toronto, Toronto, Ontario, Canada.
Biochem Biophys Res Commun ; 288(4): 990-6, 2001 Nov 09.
Article en En | MEDLINE | ID: mdl-11689007
The aryl hydrocarbon receptor (AHR) binds xenobiotic chemicals such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and regulates transcription of the P4501 subfamily that metabolizes many carcinogens. In humans, the most frequent polymorphism is R554L. We report here an additional two polymorphisms in AHR that show apparent linkage disequilibrium with the codon 554 polymorphism: the first is a previously described polymorphism, V570I; the second is a novel human AHR polymorphism, P571S. In vitro expression of these variant forms showed normal ligand binding and DNA binding activities. However, transient expression experiments revealed that the combined Ile(570) + Lys(554) variant failed to support TCDD-dependent induction of CYP1A1 expression. It is possible that the abrogation of CYP1A1 induction in the combined Lys(554) + Ile(570) variant may reduce susceptibility of the host to the carcinogenic effects of polycyclic aromatic hydrocarbons. This combination of variant genotypes is rare and appears to be confined primarily to persons of African descent.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polimorfismo Genético / Activación Transcripcional / Receptores de Hidrocarburo de Aril / Citocromo P-450 CYP1A1 Límite: Humans Idioma: En Revista: Biochem Biophys Res Commun Año: 2001 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polimorfismo Genético / Activación Transcripcional / Receptores de Hidrocarburo de Aril / Citocromo P-450 CYP1A1 Límite: Humans Idioma: En Revista: Biochem Biophys Res Commun Año: 2001 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos