Granule-dependent cytolysis of Mycobacterium tuberculosis-infected macrophages by human gammadelta+ T cells has no effect on intracellular mycobacterial viability.
Clin Exp Immunol
; 126(1): 76-83, 2001 Oct.
Article
en En
| MEDLINE
| ID: mdl-11678902
One of the most important effector functions of activated gammadelta+ T cells in tuberculosis is their strong cytolytic activity against a variety of target cells, including M. tuberculosis-infected macrophages. In the present study, we investigated the relationship between the mechanism of cytolysis utilized by gammadelta+ CTL and intracellular M. tuberculosis survival using a panel of cytolytic human M. tuberculosis-specific gammadelta+ CTL clones. Cytolysis mediated by the gammadelta+ T-cell clones was found to be Ca2+-dependent, sensitive to Cyclosporin A, and was completely abrogated following Sr2+-induced de-granulation of the gammadelta+ T cell effectors. These data demonstrate that gammadelta+ T-cell-mediated cytoxicity was mediated via the granule exocytosis/perforin pathway. Despite significant cytolytic activity against mycobacteria infected U937 cells, the gammadelta+ CTL clones had no impact on the survival of intracellular M. tuberculosis.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Tuberculosis
/
Linfocitos T Citotóxicos
/
Receptores de Antígenos de Linfocitos T gamma-delta
/
Vesículas Secretoras
/
Macrófagos
/
Mycobacterium tuberculosis
Límite:
Humans
Idioma:
En
Revista:
Clin Exp Immunol
Año:
2001
Tipo del documento:
Article
País de afiliación:
Sudáfrica
Pais de publicación:
Reino Unido