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Integrated enzyme-linked immunosorbent assay screening system for amnesic, neurotoxic, diarrhetic, and paralytic shellfish poisoning toxins found in New Zealand.
Garthwaite, I; Ross, K M; Miles, C O; Briggs, L R; Towers, N R; Borrell, T; Busby, P.
Afiliación
  • Garthwaite I; AgResearch Ruakura, Toxinology and Food Safety Group, Hamilton, New Zealand.
J AOAC Int ; 84(5): 1643-8, 2001.
Article en En | MEDLINE | ID: mdl-11601487
Enzyme-linked immunosorbent assays (ELISAs) were developed for amnesic, neurotoxic, and diarrhetic shellfish poisoning (ASP, NSP, and DSP) toxins and for yessotoxin. These assays, along with a commercially available paralytic shellfish poisoning (PSP) ELISA, were used to test the feasibility of an ELISA-based screening system. It was concluded that such a system to identify suspect shellfish samples, for subsequent analysis by methods approved by international regulatory authorities, is feasible. The assays had sufficient sensitivity and can be used on simple shellfish extracts. Alcohol extraction gave good recovery of all toxin groups. The ease of ELISAs permits the ready expansion of the system to screen for other toxins, as new ELISAs become available.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Parálisis / Mariscos / Oxocinas / Diarrea / Amnesia / Toxinas Marinas / Neurotoxinas Tipo de estudio: Diagnostic_studies / Screening_studies Límite: Animals País/Región como asunto: Oceania Idioma: En Revista: J AOAC Int Año: 2001 Tipo del documento: Article País de afiliación: Nueva Zelanda Pais de publicación: Reino Unido
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Parálisis / Mariscos / Oxocinas / Diarrea / Amnesia / Toxinas Marinas / Neurotoxinas Tipo de estudio: Diagnostic_studies / Screening_studies Límite: Animals País/Región como asunto: Oceania Idioma: En Revista: J AOAC Int Año: 2001 Tipo del documento: Article País de afiliación: Nueva Zelanda Pais de publicación: Reino Unido