Characterization of the genomic structure and tissue-specific promoter of the human nuclear receptor NR5A2 (hB1F) gene.

Zhang, C K; Lin, W; Cai, Y N; Xu, P L; Dong, H; Li, M; Kong, Y Y; Fu, G; Xie, Y H; Huang, G M; Wang, Y

Zhang, C K; Lin, W; Cai, Y N; Xu, P L; Dong, H; Li, M; Kong, Y Y; Fu, G; Xie, Y H; Huang, G M; Wang, Y.

Afiliación

Zhang CK; Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yueyang Road, 200031, Shanghai, China.

Gene ; 273(2): 239-49, 2001 Aug 08.

Article en En | MEDLINE | ID: mdl-11595170

RESUMEN

The human homologue of the Drosophila melanogaster orphan nuclear receptor fushi tarazu factor 1 (Ftz-F1), NR5A2 (hB1F), was initially identified as a regulatory factor that binds and activates enhancer II of hepatitis B virus. NR5A2 (hB1F) is expressed specifically in pancreas and liver, playing important roles in the regulation of several liver-specific genes. A detailed analysis on the genomic structure and promoter activity will greatly promote future studies on the function of the NR5A2 (hB1F) gene. In this report, a bacterial artificial chromosome clone and several phage clones covering the NR5A2 (hB1F) gene were isolated and the complete genomic sequence was obtained. Alignment of different cDNAs of the NR5A2 (hB1F) gene with the genomic sequence facilitated the delineation of its structural organization, which spans over 150 kb and consists of eight exons interrupted by seven introns. RT-PCR and 3'-RACE revealed that utilization of two polyadenylation signals results in the 3.8 and 5.2 kb transcripts that were observed previously. The transcription start site of the NR5A2 (hB1F) gene was mapped downstream of a canonical TATA box. An upstream fragment containing binding sites for several liver-specific and ubiquitous transcription factors exhibits hepatocyte-specific promoter activity. Transient transfections indicated that hepatocyte nuclear factors HNF1 and HNF3beta could activate NR5A2 (hB1F) promoter.

Asunto(s)

Proteínas de Unión al ADN; Genes/genética; Regiones Promotoras Genéticas/genética; Transactivadores/genética; Células 3T3; Animales; Secuencia de Bases; Factores de Transcripción Básicos con Cremalleras de Leucinas y Motivos Hélice-Asa-Hélice; Clonación Molecular; ADN/química; ADN/genética; Exones; Células HeLa; Factor Nuclear 4 del Hepatocito; Humanos; Intrones; Luciferasas/genética; Luciferasas/metabolismo; Ratones; Datos de Secuencia Molecular; Fosfoproteínas/genética; Fosfoproteínas/fisiología; Poli A/genética; Receptores Citoplasmáticos y Nucleares; Proteínas Recombinantes de Fusión/genética; Proteínas Recombinantes de Fusión/metabolismo; Análisis de Secuencia de ADN; Factores de Transcripción/genética; Factores de Transcripción/fisiología; Transcripción Genética; Transfección; Células Tumorales Cultivadas

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transactivadores / Regiones Promotoras Genéticas / Proteínas de Unión al ADN / Genes Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Gene Año: 2001 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos

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