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Colorimetric and gas-liquid chromatographic determination of atropine-hyoscyamine and hyoscine (scopolamine) in solanaceous plants.
J Assoc Off Anal Chem ; 58(5): 884-7, 1975 Sep.
Article en En | MEDLINE | ID: mdl-1158830
Two colorimetric micromethods are described for the determination of atropine-hyoscyamine and hyoscine (scopolamine), using p-dimethylaminobenzaldehyde and citric acid-acetic anhydride as the color reagents. These methods are sensitive to 60-1200 and 10-360 mug alkaloid/10 ml. The colorimetric methods were also successfully applied after a preliminary thin layer chromatographic separation of the alkaloids. A gas-liquid chromatographic procedure was also developed, which yielded comparable results with the colorimetric methods.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Atropina / Escopolamina / Colorimetría Idioma: En Revista: J Assoc Off Anal Chem Año: 1975 Tipo del documento: Article Pais de publicación: Estados Unidos
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Atropina / Escopolamina / Colorimetría Idioma: En Revista: J Assoc Off Anal Chem Año: 1975 Tipo del documento: Article Pais de publicación: Estados Unidos